Investigation of gut microbiome changes in type 1 diabetic mellitus rats based on high-throughput sequencing.

In this study, LEfSe multilevel species hierarchical tree directly reflects the differences of gut microbiota between control and T1DM rats at the Phylum-to-Family level, the result showed that the gut microbiome of T1DM rats changed significantly. The abundance of pathogenic bacteria associated with infection and inflammation in T1DM rats was up-regulated such as Ruminococcaceae, Shigella, Enterococcus, Streptococcus, Rothia and Alistipes, while the abundance of beneficial bacteria and bacteria producing SCFAs were reduced such as Lactobacillus, Faecalitalea, Butyricicoccus and Allobaculum.