Construction and heterologous overexpression of two chimeric proteins carrying outer hydrophilic loops of Vairimorpha ceranae and Nosema bombycis ATP/ADP carriers.

Viacheslav V Dolgikh,Sergey A Timofeev,Vladimir S Zhuravlyov,Igor V Senderskiy

Journal of Invertebrate Pathology（2020）

引用 3|浏览6

暂无评分

摘要

•Microsporidia ATP/ADP carriers possess 6 outer, 7 inner and 12 transmembrane domains;•Outer loops of 4 Vairimorpha ceranae and 3 N. bombycis carriers were fused in two proteins;•Chimeric proteins and fused outer loops of individual carriers were overexpressed in E. coli;•Chimeric antigens may be used to construct and select microsporidia-suppressing single-chain antibodies.

查看译文

关键词

Domesticated insects,Microsporidiosis,Outer loops of ATP/ADP carriers,Chimeric proteins,Heterologous expression,Antibody libraries

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要