FABRICATION, EVALUATION OF DRUG LOADING CAPABILITY AND CHARACTERIZATION OF 3D-NANO-CELLULOSE NETWORK MATERIALS PRODUCED BY BACTERIA OF FERMENTED AQUEOUS GREEN TEA EXTRACTIN SELECTED CULTURE MEDIA

Objective: The study aims for the fabrication, evaluation of drug loading capability and characterization of 3D-nano-cellulose network materials produced by bacteria of fermented aqueous green tea extract in selected culture media. Methods: 3D-nano-cellulose network (3DNC) materials can be produced by bacteria living in a fermented aqueous green tea extract. 3DNCs include nano-fibers forming networks, which are capable of drug loading to form a prolonged release therapy to improve drug bioavailability. In this study, 3DNC materials are biosynthesized by aerobic bacteria in the standard medium (SM), coconut water (CW) and rice water (RW). 3DNCs were prepared and evaluated for drug carrier using famotidine as a model drug. Famotidine was loaded in 3DNC by the absorption method. 3DNCs were characterized by using FE-SEM and FTIR spectroscopy. Results: The 3DNCs obtained from CW, and RW have the same characteristics as the 3DNC obtained from the SM, and 3DNCs can be fabricated with the desired thickness and diameter in all three types of culture media. 3DNCs absorbed famotidine in optimum condition without any difference in famotidine loading (28.2 mg) and famotidine entrapment efficiency (90 %). Investigation of the 3DNC structure using FE-SEM has shown that the cellulose fibers of 3DNC-SM and 3DNC-CW have a stable structure without structural change when loading drug under optimal condition. Conclusion: The results indicate the potential for using 3DNC-SM and 3DNC-CW to design the drug delivery system.