Comparative Efficacy of Bacillus thuringiensis Commercial Formulations against Leaf Worm, Spodoptera litura Fabricius under Laboratory Conditions
PAKISTAN JOURNAL OF ZOOLOGY(2020)
摘要
A study was carried out in the Bio-control Laboratory, Department of Entomology at Pir Mehr Ali Shah, Arid Agriculture University Rawalpindi to check the efficacy of commercial biopesticides under controlled environmental conditions. Bio pesticides are important alternates for chemical control of economically damaging insect pests like leaf worm, Spodoptera litura Fabricius. In this study, two commercial products including Dipel withBt sub speciess kurstaki and Turex withBt sub speciess kurstaki and aizawai were tested against three early larval instars of S. litura under laboratory conditions using leaf dip method. Mortality was recorded after three and seven days of exposure. The results indicated that larval mortality increased with time and Turex (Bt sub speciess kurstaki and aizawai) after 3 days of exposure caused significantly higher mortality i.e 46.43, 43.45 and 38.69 % as compared to Dipel (Bt sub speciess kurstaki) that caused 19.05, 6.55 and 4.76 % mortality for 1", 2nd and 3rd instar, respectively. The data for 7th day also showed significantly higher mortality as 64.29, 60.71 and 45.24 % by Turex (Bt sub speciess kurstaki and aizawai) in comparison with 55.95, 57.74 and 42.86 % mortality by Dipel (Bt sub species kurstaki) for 1", 2nd and 3rd instar, respectively. Susceptibility to both bio pesticides increased with increase in their concentration and decreased with increase in larval instar. Similarly LC5o values suggested Turex (Bt sub species kurstaki and aizawai) to be more toxic with less LC5o values as compared to Dipel (Bt sub species kurstaki). These results indicated that these bio pesticides if used at early insect stage can help to control this pest.
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关键词
Spodoptera litura,Bacillus thuringiensis,Commercial formulations,Laboratory bioassays
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