Pharmacological Study of Carbonized Scutellaria Radixin Treating Abnormal Uterine Bleeding by Spectrum-Effect Relationship

Background and Objective: Carbonized Scutellaria Radix (Carbonized Huangqin) is a kind of processed products of Scutellaria baicalensis, which was reported to possess the therapeutic effect of gynecological hemorrhage but the material basis is not clear. The purpose of this study was to study the anti-abnormal uterine bleeding (AUB) effect and chemical markers of Carbonized Huangqin. Materials and Methods: In this study, 15 batches of Scutellaria baicalensis collected from different origins were processed into carbonized forms. The HPLC-DAD fingerprint was developed by the optimized chromatographic condition, similarity analysis (SA) and principal components analysis(PCA) were used to evaluate the different samples. Experiment model of abnormal uterine bleeding (AUB) in rats was established to estimate the effect of Carbonized Huangqin. Gray relational analysis (GRA) and orthogonal partial least squares discrimination analyze (OPLS-DA) were applied to analyze the spectrum-effect relationship between HPLC fingerprints and haemostatic effect of Carbonized Huangqin on AUB in rats. Results: The similarities of all samples were more than 0.9. Spectrum-effect relationship revealed that peak 13 (wogonin), 14 (chrysin), 15 (oroxylin-A), 5 (baicalein-7-O-D-glucoside) and 6 (baicalin) were related to haemostatic effect, they were arranged in the following order: wogonin>chrysin>oroxylin-A>baicalein-7-O-D-glucoside>baicalin. Conclusion: Wogonin, chrysin, oroxylin-A might be the most likely chemical markers of Carbonized Huangqin in the treatment of AUB.