Isolation and genetic characterization of Neospora caninum from naturally infected sheep.

•In vitro isolation and genotyping of two new Neospora caninum isolates is described.•Nc-Spain11 and Nc-Spain12 were isolated from two congenitally infected lambs.•Neospora caninum ovine isolates were implicated in reproductive failure in a flock.•Both isolates showed identical multilocus microsatellite genotype.•Ovine genotype showed close genetic relationship with bovine genotypes.