Gene Silencing by RNAi in Mammalian Cells.

Frida Ponthan,Narazah Mohd Yusoff,Natalia Martinez Soria,Olaf Heidenreich,Kelly Coffey

Current protocols in molecular biology（2015）

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摘要

This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.

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关键词

RNA interference,electroporation,lentiviral transduction,shRNA,siRNA,transfection

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