MULTIRESOLUTION APPLICATION OF ARTIFICIAL INTELLIGENCE IN DIGITAL PATHOLOGY FOR PREDICTION OF POSITIVE LYMPH NODES FROM PRIMARY TUMORS IN BLADDER CANCER

PURPOSE To develop an artificial intelligence (AI)-based model for identifying patients with lymph node (LN) metastasis based on digital evaluation of primary tumors and train the model using cystectomy specimens available from The Cancer Genome Atlas (TCGA) Project; patients from our institution were included for validation of the leave-out test cohort.METHODS In all, 307 patients were identified for inclusion in the study (TCGA, n=294; in-house, n=13). Deep learning models were trained from image patches at 2.5x, 5x, 10x, and 20x magnifications, and spatially resolved prediction maps were combined with microenvironment (lymphocyte infiltration) features to derive a final patient-level AI score (probability of LN metastasis). Training and validation included 219 patients (training, n=146; validation, n=73); 89 patients (TCGA, n=75; in-house, n=13) were reserved as an independent testing set. Multivariable logistic regression models for predicting LN status based on clinicopathologic features alone and a combined model with AI score were fit to training and validation sets.RESULTS Several patients were determined to have positive LN metastasis in TCGA (n=105; 35.7%) and inhouse (n=3; 23.1%) cohorts. A clinicopathologic model that considered using factors such as age, T stage, and lymphovascular invasion demonstrated an area under the curve (AUC) of 0.755 (95% CI, 0.680 to 0.831) in the training and validation cohorts compared with the cross validation of the AI score (likelihood of positive LNs), which achieved an AUC of 0.866 (95% CI, 0.812 to 0.920; P=.021). Performance in the test cohort was similar, with a clinicopathologic model AUC of 0.678 (95% CI, 0.554 to 0.802) and an AI score of 0.784 (95% CI, 0.702 to 0.896; P=.21). In addition, the AI score remained significant after adjusting for clinicopathologic variables (P=1.08 x 10(-9)), and the combined model significantly outperformed clinicopathologic features alone in the test cohort with an AUC of 0.807 (95% CI, 0.702 to 0.912; P=.047).CONCLUSION Patients who are at higher risk of having positive LNs during cystectomy can be identified on primary tumor samples using novel AI-based methodologies applied to digital hematoxylin and eosin-stained slides. (c) 2020 by American Society of Clinical Oncology