Taxotere-induced WNT16 Expression in Carcinoma-Associated Fibroblasts Might Associate with Progression and Chemoresistance of Breast Cancer.
ANNALS OF CLINICAL AND LABORATORY SCIENCE(2020)
摘要
Objective. The objective of this study is to investigate the relationship between WNT16 expression in carcinoma-associated fibroblasts and Taxotere treatments in breast cancer. Procedures. WNT16 expression in primarily cultured carcinoma-associated fibroblasts was detected via Real-Time PCR and Western blot. A total of 106 breast cancer cases were prepared for immunohistochemistry. Multiple assays were performed to examine the function of breast cancer cells (MDA-MB-231) co-cultured with carcinoma-associated fibroblasts when WNT16 was inhibited. Results. Enhanced WNT16 expression was verified in carcinoma-associated fibroblasts after Taxotere treatment (p<0.0001). MDA-MB-231 cells co-cultured with carcinoma-associated fibroblasts displayed decreasing invasion, proliferation abilities, and an increasing sensitivity to Taxotere treatment when WNT16 was inhibited. The WNT16 expression of carcinoma-associated fibroblasts in breast cancer was significantly associated with tumor size (p=0.0164), and chemotherapy treatment (p=0.0015). Conclusions. The results indicate that Taxotere-induced WNT16 expression in carcinoma-associated fibroblasts might contribute to the progression and chemoresistance of breast cancer.
更多查看译文
关键词
WNT16,carcinoma-associated fibroblasts,breast cancer,chemoresistance
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要