Identification of six important amino acid residues of MenA from Bacillus subtilis natto for enzyme activity and formation of menaquinone

Liu-xiu Hu,Jing-jing Feng,Wu Jing,Wei Li, Sokhna mbacke Gningue,Zi-ming Yang,Wang Zhou,Zheng-lian Xue, Yan Liu

Enzyme and Microbial Technology（2020）

引用 9|浏览67

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摘要

•Four Asp sites are vital for 1, 4-dihydroxy-2-naphthoic acid prenyltransferase (MenA).•Two sites (Q67th, N74th) positively affect MenA activity and menaquinone titer.•Mutant which Q67th was changed to R67th in MenA results a 5-fold increase in YP1/x.•Hydrophobic interaction was beneficial for promoting MenA activity.

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关键词

1,4-dihydroxy-2-naphthoic acid (DHNA),DHNA prenyltransferase (MenA),Bacillus subtilis natto,Menaquinone,Site-directed mutagenesis

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