Terrilactibacillus Tamarindi Sp. Nov., Isolated from Bark of Tamarindus Indica

A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1(T), was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand. This strain produced o-lactic acid from glucose. It grew at 20-45 degrees C (optimum, 30 degrees C), pH 3.5-9 (optimum, pH 7.0) and in the presence of 1-4 % (w/v) NaCl. The cell-wall peptidoglycan contained meso-diaminopimelic acid (A1 gamma). The major isoprenoid quinone was menaquinone 7 (MK-7). Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid. The predominant cellular fatty acids were anteiso-C-17:0, anteiso-C-15:0, and iso-C-16:0 when cultivated on GYP agar plates. The 16S rRNA gene sequence similarity between strain BCM23-1(T) and Terrilactibacillus laevilacticus NK26-11(T) was 98.3 %. The draft genome of BCM23-1(T) was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%. The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1(T) and T. laevilacticus NK26-11Twere 89.9, 90.8 and 40.4 %, respectively. The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1(T) as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp. nov. is proposed. The type strain is BCM23-1(T) (=LMG 31662(T)=JCM 33748(T)=TISTR 2841(T)).