Nqo1 Downregulation Generates Genotoxic Dna Adducts In In Vitro Fecd Model

Purpose: Fuchs Endothelial Corneal Dystrophy (FECD) is a late-onset oxidative stress disorder characterized by progressive loss of corneal endothelial cells (CEnCs). FECD has a greater incidence in women at a ratio of 4: 1 suggesting the possible role of sex hormones accounting for predominance in females. The aim of this study is to investigate the role of NAD (P) H: quinone oxidoreductase 1 (NQO1) downregulation in generating genotoxic estrogen metabolites in FECD.Methods: Cell viability by Cell Titer Glo, apoptotic cell population by Annexin-V (Ann) and propidium iodide (PI) staining using flow cytometry and estrogen metabolites by ultraperformance liquid chromatography/tandem mass spectrometry (UPLC/MS) were investigated in immortalized normal (HCEnC-21T, HCEnC-SV-67F-16, NQO1-WT and KO) and FECD (FECD-SV-73F-74 and-61F-18) human CEnC lines treated with and without …