Sustainable DNA data storage with low biased manipulation

In DNA data storage, the huge sequence complexity is challenging repeatable and efficient information reading. Here, we demonstrated that biased amplification is serious matter for stable information retrieve from synthetic oligo pool comprising over ten thousand strands encoding over 2.85 MB digital information. Therefore, we adapted isothermal DNA amplification as a new biochemical framework, termed as isothermal DNA reading (iDR) for low biased manipulation of DNA pool with huge sequence complexity. iDR was built to work in a priming-free and error-spreading proof manner and achieved low-biased amplification. Finally, we demonstrated that immobilized DNA materials read by iDR is able to stably read data deeply and repeatedly, the first reported sustainable DNA storage system. Necessary amount of sequencing reads for perfect oligos retrieve significantly decreased. These advanced features enable the iDR chemical framework being ideal alternative for manipulating the huge sequence complexity and building the sustainable and efficient DNA storage "hardware".