(Peer Review) The use of probiotic isolated from Sangkuriang catfish (Clarias gariepinus var. Sangkuriang) intestine to improve growth and feed efficiency of carp, Cyprinus carpio

The effect of probiotic isolated from sangkuriang catfish (Clarias gariepinus var. Sangkuriang) on growth and feed efficiency of carp (Cyprinus carpio) has been studied. Carp juveniles weighing 2.13±0.09 g in average were obtained from Freshwater Aquaculture Center Tatelu, North Minahasa Regency. After acclimatization in laboratory condition for two weeks, the juveniles were cultivated in 15 aquaria with a density 15 individuals each. Probiotic used was obtained from the Laboratory of Fish Health, Environment and Toxicology of the Faculty of Fisheries and Marine Science Sam Ratulangi University Manado-Indonesia. Fish were fed with feed added with probiotic bacteria with different concentrations namely 1x10, 1x10, 1x10, 1x10 cfu mL. Control food was not supplemented with probiotic. Probiotic-feed was applied for 21 days at 5% of their body weight per day, twice daily. The data collected consisted of average daily growth rate, weight gain, feed efficiency and feed conversion ratio. The results showed that the addition of probiotic bacteria in feed was able to increase the growth, feed efficiency and decrease the feed conversion ratio (p < 0.01). The best growth, feed efficiency and feed conversion ratio were achieved in fish fed with the addition of probiotic bacteria 1x10 cfu mL. In conclusion, probiotic bacteria isolated from catfish intestine have the potential to improve the growth performance, feed efficiency and decrease feed conversion ratio. The best results were observed in fish fed food supplemented with probiotic suspension containing 1x10 cfu mL.