Effect of Ethyl-p-Methoxy Cinnamate from Kaempferia galanga on Cytochrome P450 Enzymes Expression in Mouse Hepatocytes

Kaempferia galanga, a plant in the Zingiberaceae family, has been widely used as a spice, food flavoring and folk medicine. Its rhizome contains ethyl-p-methoxycinnamate (EPMC) as a major constituent. This study aimed to investigate the effect of EPMC on cytochrome P450 expression in mouse primary hepatocytes. EPMC was isolated from the dichloromethane extract of dried rhizome of K. galanga by column chromatographic method. For cytotoxicity test, EPMC at a concentration of 2.42 mM is relatively non-toxic to primary cultured hepatocytes. EPMC at this concentration induced CYP1A1 and CYP1A2 mRNA expressions in the mouse hepatocytes. However, EPMC at this concentration did not show significant induction of CYP2B9 and CYP3A11 naRNA expressions. Moreover, EPMC can modulate the inductive expression of CYP1A1 mRNA by specific inducers, B[a]A and beta-NF. In which the synergistic effects were observed at 72 and 48 h for B[a]A and beta-NF treatments, respectively Therefore, the consumption of K. galanga may be potentially caused herbal-drug or herbal-chemical interactions.