Quantitative Determination of the Main Substance of the Triazid® Antiviral Drug by Voltammetry

The new Russian drug Triazid® is a highly effective antiviral agent. The active substance of the drug (5-methyl-6-nitro-7-oxo-4,7-dihydro-1,2,4-triazolo[1,5-a] pyrimidinide l-arginine monohydrate, triazid) belongs to azoloazine series compounds. Using cyclic voltammetry, it was found that the electrochemical activity of triazid (TD) in Britton–Robinson aqueous buffer solutions (BRB) on a glassy carbon electrode is due to the electroreduction of the nitro group bound with the conjugated aromatic system. The maximum current of triazid reduction in the potential range 0.0‒(‒1.25) V was used as an analytical signal. A method was developed for the quantitative determination of triazid by direct cathodic square-wave voltammetry. The linearity range of the calibration graph using the developed method in a BRB solution (pH 4) is 10–500 mg/L (R2 = 0.9998) with the limit of detection for TD 1.9 mg/L. The developed method was used to analyze the Triazid pharmaceutical substance for the concentration of TD. The results obtained satisfy the stated requirements of the draft pharmacopoeial article of the company “TRIAZID substance” for the content of TD found by HPLC.