FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants.
CHEMICAL COMMUNICATIONS(2020)
摘要
Herein, we report a FnCas12a/crRNA assisted Dumbbell-PCR method for the detection of isomiRs with double specificity and magnification. The single nucleotide variant of isomiRs in terminals and/or inner sequence could be discriminated by this strategy. Using this method, let-7a isomiRs in MCF-7 and MCF-7R cell lines were analyzed.
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