Enhancement of Culture of Legionella Longbeachae from Respiratory Samples by Use of Immunomagnetic Separation and Antimicrobial Decontamination.
Journal of clinical microbiology(2020)
摘要
Legionella longbeachae is the commonest Legionella species identified in patients with community-acquired pneumonia in New Zealand. Isolation of the organism on culture is the gold standard for the diagnosis of Legionnaires disease, but it has poor sensitivity (40%) compared with quantitative PCR (qPCR). We have developed a selective decontamination process using glycine, vancomycin, polymyxin, and cycloheximide (GVPC) with immunomagnetic separation (IMS) for culturing L. longbeachae .
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关键词
Legionella,clinical methods,immunomagnetic separation
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