Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples

Marianoel Pereira-Gómez,Álvaro Fajardo,Natalia Echeverría, Fernando López-Tort,Paula Perbolianachis, Alicia Costábile,Fabián Aldunate,Pilar Moreno,Gonzalo Moratorio

Journal of Virological Methods（2021）

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摘要

•A SYBR Green assay to detect SARS-CoV-2 based on WHO protocols was performed.•The analytical sensitivity of this method was comparable with the reference assay.•SYBR Green assay represents a reliable cost-effective alternative for testing.

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关键词

SARS-CoV-2,Molecular detection,SYBR Green,RT-qPCR

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