Loss Ofcntnap2in The Rat Causes Autism-Related Alterations In Social Interactions, Stereotypic Behavior, And Sensory Processing

Autism spectrum disorder (ASD) is characterized by social interaction and communication impairments, as well as restrictive/repetitive patterns of behavior, interests or activities, which can coexist with intellectual disability and altered sensory processing. To study the mechanisms underlying these core features of ASD, preclinical research has developed animal models with manipulations in ASD-linked genes, such asCNTNAP2. In order to fully interpret the findings from mechanistic studies, the extent to which these models display behaviors consistent with ASD must be determined. Toward that goal, we conducted an investigation of the consequences of a functional loss ofCntnap2on ASD-related behaviors by comparing the performance of rats with a homozygous or heterozygous knockout ofCntnap2to their wildtype littermates across a comprehensive test battery.Cntnap2(-/-)rats showed deficits in sociability and social novelty, and they displayed repetitive circling and hyperlocomotion. Moreover,Cntnap2(-/-)rats demonstrated exaggerated acoustic startle responses, increased avoidance to sounds of moderate intensity, and a lack of rapid audiovisual temporal recalibration; indicating changes in sensory processing at both the pre-attentive and perceptual levels. Notably, sensory behaviors requiring learned associations did not reveal genotypic differences, whereas tasks relying on automatic/implicit behaviors did. Ultimately, because these collective alterations in social, stereotypic, and sensory behaviors are phenotypically similar to those reported in individuals with ASD, our results establish theCntnap2knockout rat model as an effective platform to study not only the molecular and cellular mechanisms associated with ASD, but also the complex relationship between altered sensory processing and other core ASD-related behaviors. Lay Summary Autism spectrum disorder (ASD) is characterized by social interaction differences, and restrictive/repetitive patterns of behavior. We studied the behavioral alterations caused by the loss of an autism-linked gene,Cntnap2, in the rat to determine how mutations in this gene contribute to autism-related behaviors. We show the loss ofCntnap2leads to changes in social, stereotypic, and sensory behaviors, indicating this rat model can be used to better understand the brain changes underlying ASD.