A novel microfluidic paper-based analytical device based on chemiluminescence for the determination of β-agonists in swine hair.

beta-Agonists are illegal feed additives in the feed industries of many countries, especially China. Here, we report a microfluidic paper-based analytical device (mu PAD) coupled with the chemiluminescence (CL) method to provide the sensitive, simple and rapid quantitative detection of beta-agonists in swine hair samples. In this study, we found that the beta-agonists diminished the CL generated by the reaction of K-3[Fe(CN)(6)] and luminol on mu PAD, which was different from that observed in the aqueous solution, and the degree of diminishment was proportional to the concentration of beta-agonists. The possible mechanism was discussed as well. Also, this detection method showed a wide linear range (from 4.0 x 10(-8) to 1.0 x 10(-5) mol L-1) and low limit of detection (2.0 x 10(-8) mol L-1) with a low consumption of samples and reagents. Satisfactory recovery values (from 78% to 95%) were achieved. Therefore, our mu PAD CL sensor will be favorable to develop a miniaturized instrument for the on-site analysis of beta-agonists in swine hair samples.