Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition
EPITHELIAL-TO MESENCHYMAL TRANSITION: Methods and Protocols(2021)
摘要
Molecular Tension Microscopy has been increasingly used in the last years to investigate mechanical forces acting in cells at the molecular scale. Here, we describe a protocol to image the tension of the junctional protein E-cadherin in cultured epithelial cells undergoing Epithelial-Mesenchymal Transition (EMT). We report how to prepare cells and induce EMT, and how to acquire, analyze, and quantitatively interpret FRET data.
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关键词
FRET Biosensor,E-cadherin,EMT,Microscopy,Mechanotransduction
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