The accumulation of colibactin intermediates does not affect the growth and morphology of pks + Escherichia coli

Colibactin is a natural product made by numerous strains of that harbor the genomic island. The deletion of one of the genes within the the peptidase clbP, has been found to disrupt the maturation of colibactin, thus promoting the accumulation in the periplasmic space of numerous biosynthesis intermediates, some of which have been characterized chemically. To date, no one has reported the effect of such an accumulation of intermediates on the cellular morphology of the producing bacterium. In this report, we describe the scanning electron microscopy (SEM) images of numerous clinical isolates of harboring the , collected from Puerto Rico hospitals. We have observed that the wild type isolates that harbor the display lesions on the bacterial envelope surface. These lesions are absent in isolates lacking the . To determine whether this phenotype is associated with colibactin production, we deleted the gene from the extraintestinal pathogenic strain IHE3034, thus disrupting its ability to make colibactin. The wild-type IHE3034 displayed a spherical shape with no envelope lesions, and was practically indistinguishable from the Δ deletion mutant. To our knowledge, this work provides the first SEM images of a pks deletion mutant.