Usp44hypermethylation Promotes Cell Proliferation And Metastasis In Breast Cancer

Aim:The methylation and expression levels ofUSP44in breast cancer were investigated and their effects on tumor cells were researched.Materials & Methods:Bioinformatics was employed to identify the target gene from TCGA database. Sodium bisulfite and decitabine were used for DNA modification and demethylation, and methylation-specific PCR and reverse transcriptase PCR were performed to assessUSP44methylation and expression levels. Tumor cell behaviors were assayed via severalin vitroexperiments.Results:USP44was hypermethylated, which caused its poor expression in breast cancer, whereas its overexpression significantly suppressed cancer cell proliferation, migration and invasion and induced apoptosis.Conclusion:USP44negatively functions in cancer progression upon overexpression, indicating its potential as a therapeutic target for clinical treatment of breast cancer.