Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis
DEVELOPMENTAL BIOLOGY OF THE SEA URCHIN AND OTHER MARINE INVERTEBRATES, 2 EDITION: Methods and Protocols(2021)
摘要
Animal regeneration is a biological process leading to the reformation of injured or lost tissues/body parts. One of the most fascinating regenerative phenomena is the so-called whole-body regeneration, leading to the reformation of fully functional organisms within days after bisection. The sea anemone Nematostella vectensis is currently emerging as novel whole-body regeneration model. Here we describe the methods of inducing the regenerative process in this cnidarian as well as the fixation and staining protocols for morphological, molecular, and cellular analysis.
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关键词
Whole body regeneration,Bisection,Fixation,Permeabilization,Immunohistochemistry,Staining
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