In Silico Identification of Cytochrome P450s Involved in Ocimum Tenuiflorum Subjected to Four Abiotic Stresses

A wide array of biosynthetic reactions involves plant cytochrome P450s leading to the production of different biomolecules, that include diverse defense compounds. The present study is an effort to provide the basis for the future studies on CYP450 functional characterization that might help improve stress tolerance in Ocimum tenuiflorum against severe cold, flooding, drought and salinity. On the whole, in this study CYP450 transcripts from the RNA sequencing data of O. tenuiflorum under cold, flood, drought and salinity stresses were analysed. These transcripts got categorized into CYP51, CYP71, CYP72, CYP73, CYP74, CYP75, CYP76, CYP77, CYP78, CYP79, CYP81, CYP82, CYP83, CYP84, CYP85, CYP86, CYP88, CYP90, CYP94, CYP96, CYP97, CYP98, CYP701, CYP703, CYP704, CYP707, CYP709, CYP710, CYP711, CYP714, CYP716, CYP724, CYP734, and CYP735 clans. Maximum number of transcripts in all the stresses belonged to CYP76, CYP75 and CYP716 families which are related to flavonoid and triterpenoid metabolism. CYP450s among all the abiotic stresses were most affected in the flooding stress followed by cold, drought and salinity.