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Brief comparative evaluation of six open one step RT qPCR mastermixes for the detection of SARS CoV 2 RNA using a Taqman probe

Cyrille Haddar,Paul O. Verhoeven,Thomas Bourlet,Bruno Pozzetto,Sylvie Pillet

Journal of Clinical Virology（2020）

Cited 4|Views22
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Abstract
•In case of virus emergence, laboratory-developed assays (LDA) should be urgently assessed.•Recommended open one-step RT-qPCR reagents are rapidly on tension in case of pandemic.•Five other reagents were shown to be used for SARS-CoV-2 RNA detection by LDA.•This study can help laboratories to assess LDA for detecting emerging RNA viruses.
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Key words
Coronavirus,SARS-CoV-2,RNA virus,Emerging virus,Laboratory-developed assay,RT-qPCR
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