Enzymatic preparation of pyruvate by a whole-cell biocatalyst coexpressing l-lactate oxidase and catalase

Guosi Li,Jiazhang Lian,Hailong Xue,Yiqi Jiang,Mianbin Wu,Jianping Lin,Lirong Yang

Process Biochemistry（2020）

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摘要

•Conversion of l-lactate was significantly improved in the cascade system.•Coexpression cassette was fine-tuned via ribosome binding site engineering.•Recombinational Escherichia coli was first constructed for biocatalytic pyruvate production.

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关键词

Lactate oxidative dehydrogenation,Biocatalyst,Lactate oxidase,Catalase coexpression

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