Intensifying Treatment in Childhood B-Lymphoblastic Leukemia with IKZF1 Deletions Negates Its Adverse Outcome: Results of Ma-Spore ALL 2003 and 2010 Studies

Deletion of IKZF1 (IKZF1de l) is a recently described adverse prognostic factor in childhood B-lymphoblastic leukemia (B-LL). In an attempt to negate the adverse effect of IKZF1 del the Ma-Spore ALL 2010 (MS2010) protocol upgraded patients to the next higher treatment risk group if they carry IKZF1 del. Specifically, patients with IKZF1 del are assigned to a risk group one tier higher, (i.e. SR upgraded to IR and IR upgraded to HR accordingly). Risk stratification criteria were otherwise similar between MS2003 and MS2010. IKZF1 status was determined using SALSA MLPA P335 kit (MRC-Holland) for 410 and 275 B-LL patients treated on MS2003 and MS2010 protocols respectively. The two cohorts were similar in presenting clinical features and genetic subtypes. IKZF1de l included any type of intragenic deletion or the loss of entire locus, and was compared to the “No-Del” group. IKZF1 del-plus group was recently described and was defined as the co-deletion of IKZF1 and at least one of CDKN2A / CDKN2B, PAX5 or BTG1 gene in this study. The differences in 5-yr cumulative incidence of relapse (5-yr CIR) were assessed using the Gray test (univariate) or competing-risks regression based on Fine and Gray's proportional subhazards model (multivariate). IKZF1 del was found in 15.9% of entire group and 13.2% of BCR - ABL 1-negative cases. IKZF1 del was overrepresented in BCR - ABL 1-positive (70.6%) and B-other (21.8%). Compared with those without IKZF1 del, patients with IKZF1 del were older (≥10 yrs; P In MS2003, the overall 5-yr CIR in IKZF1 del was significantly higher at 30.4% compared to 8.1% in No-Del group (P In MS2010, the overall 5-yr CIR in IKZF1 del improved to 9.5% compared to 4.3% in No-Del group (P=0.111; Figure 1c). This is a significant improvement when compared to MS2003 (9.5% vs. 30.4%, P=0.022; Figure 1d). The overall 5-yr CIR in patients with IKZF1 del-plus also dropped from 40.0% to 17.3%. In addition, patients with IKZF1 del no longer showed significantly worse outcome in any Day 33 MRD risk groups. When compared to the corresponding MRD risk group in MS2003, 5-yr CIR dropped from 7.7% to 0 in MRD-LR, from 43.5% to 18.8% in MRD-IR, and from 50.0% to 10.0% in MRD-HR. The 5-year overall survival for patients with IKZF1 del was also improved from 69.6% in MS2003 to 90.6% in MS2010 (P=0.012), indicating that intensified treatment did not lead to higher incidence of toxic death. In conclusion, our study shows that upgrading the treatment risk group for children with B-LL and IKZF1 del negates the adverse outcome of IKZF1de l particularly for Day 33 MRD-positive (≥10-4) patients. Download : Download high-res image (203KB) Download : Download full-size image Disclosures No relevant conflicts of interest to declare.