GROWTH-RELATED EXPRESSED SEQUENCE TAG - SAMPLE SEQUENCE REPEATS (EST-SSRS) SCREEN OF MUD IVORY WHELK (BABYLONIA LUTOSA) THROUGH TRANSCRIPTOME SEQUENCING

Mud ivory whelk (Babylonia lutosa) is one of the most economically important seawater mollusks in China However, currently, there is no report on the molecular markers related to the growth of Babylonia lutosa, which hinders the its genetic breeding with dominant growth traits. The present study was the first to sequence the transcriptomes of B. lutosa, and then searched for EST-SSR in the growth-related genes. Wild B. lutosa samples were collected from the Lianjiang River, Fujian Province of China The RNA of gastropods and hepatopancreas tissues from three B. lutosa were measured and mixed, and sequenced by Illumina HiSeq sequencer. A total of 24,829,460 reads containing 5,015,236,064 bp were obtained through sequencing. Eight growth-related genes (TBC-1-A, TBC-1-2B, FGFR-1, GFRBP2, GFR-15, FGFR-1-A, SMAD6, and TGFOR-1) containing EST-SSR sequences were detected in the B. lutosa transcriptomes. Our results would provide theoretical basis for B. lutosa breeding based on growth-related EST-SSRs.