Hydrogen Sulfide-Evoked Intracellular Ca 2+ Signals in Primary Cultures of Metastatic Colorectal Cancer Cells.

Exogenous administration of hydrogen sulfide (HS) is emerging as an alternative anticancer treatment. HS-releasing compounds have been shown to exert a strong anticancer effect by suppressing proliferation and/or inducing apoptosis in several cancer cell types, including colorectal carcinoma (CRC). The mechanism whereby exogenous HS affects CRC cell proliferation is yet to be clearly elucidated, but it could involve an increase in intracellular Ca concentration ([Ca]). Herein, we sought to assess for the first time whether (and how) sodium hydrosulfide (NaHS), one of the most widely employed HS donors, induced intracellular Ca signals in primary cultures of human metastatic CRC (mCRC) cells. We provided the evidence that NaHS induced extracellular Ca entry in mCRC cells by activating the Ca-permeable channel Transient Receptor Potential Vanilloid 1 (TRPV1) followed by the Na-dependent recruitment of the reverse-mode of the Na/Ca (NCX) exchanger. In agreement with these observations, TRPV1 protein was expressed and capsaicin, a selective TRPV1 agonist, induced Ca influx by engaging both TRPV1 and NCX in mCRC cells. Finally, NaHS reduced mCRC cell proliferation, but did not promote apoptosis or aberrant mitochondrial depolarization. These data support the notion that exogenous administration of HS may prevent mCRC cell proliferation through an increase in [Ca], which is triggered by TRPV1.