REGENERATION OF PLANTS FROM ANCESTRY TREE OF CITRUS CHANGSHAN-HUYOU YB CHANG VIA TISSUE CULTURE

Tissue culture with young leaves, stems, and embryos of the commonly grown cultivars of Citrus changshan-huyou Y.B. Chang was carried out. The results indicated that the young embryo of C. changshanhuyou cultivars was the best explant for regeneration of plants via tissue culture. Based on this result, young fruit of "Ancestry Tree" of Citrus changshan-huyou was selected. After being stored at 4 degrees C for 6 days, the embryos were cultured on MS medium containing 0.5 mg/l BA + 0.5 mg/l NAA to induce the formation of callus. The induced callus were cultured on MS medium containing 1 mg/l BA and induced to differentiate into cluster buds, which were cut and cultured on MS medium containing 0.4 mg/l IAA to induce the formation of roots. Finally, a technological system for regeneration of plants from the "Ancestry Tree" of C. changshan-huyou via tissue culture was successfully established. This study has paved the foundation for protection and further utilization of "Ancestry Tree" of C. changshan-huyou, the precious and rare germplasm resource.