Fractionation of Azaphilones from Secondary Metabolites of Marine-Derived Fungus Penicillim Pinophilum and Their Antibacterial Activity

Bioassay-guide fractionation of the marine. derived fungus Penicillim pinophilum led to the isolation of three azaphilones derivatives: Pinophilins G(1), Pinophilins B(2) and Sch 725680(3), which were separated by silica gel column chromatography, octadecylsilyl(ODS) column chromatography, Sephadex LH-20. Compound 1 is a new compound. The plane structure of compound 1 was determined based on HR-ESI-MS, H-1 NMR, C-13 NMR, heteronuclear multiple bond correlation(HMBC), nuclear overhauser effect spec. troscopy(NOSEY) and heteronuclear singular quantum correlation(HSQC). The absolute configuration of compound 1 was determined by comparing its experimental electronic circular dichroism(ECD) with the calculated ECD spectra using quantum methods. The results indicated that compounds 1 and 3 showed antibacterial activities against four spices of pathogenic bacteria.