SYNERGY OF GREEN TEA REDUCED TAMOXIFEN-LOADED SILVER NANOPARTICLES EXHIBIT OGT DOWNREGULATION IN BREAST CANCER CELL LINE

Breast cancer is the most prevalent type of cancer diagnosed in women. Current therapeutic methodologies for cancer include radiotherapy, chemotherapy and surgery. However, the major clinical obstacle in the treatment of cancer with chemotherapy is the development of de novo or acquired drug resistance due to ineffective drug delivery to the tumor site or the development of resistance with passage of treatment. To overcome these limitations, therapeutic nanoparticles have emerged as auspicious alternative of the conventional chemotherapy against breast cancer drug resistance and demonstrate improved efficacy with reduced adverse effects. Therefore it is important to comprehend the underpinning mechanism of nanoparticles interaction with cancer cells to figure out the lethal effect of nanoparticles on otherwise healthy cells. In this study, we synthesized therapeutic silver nanoparticles by the reduction of metal salt through tea extract in the presence of Tamoxifen. The cytotoxicity of Tamoxifen capped green tea reduced nanoparticles (TAM-AgNPs) was found to increase and the effect was co-related with decreased expression of an enzyme, called O-GlcNAc Transferase (OGT). This suggests that, OGT can be targeted as potential therapeutic candidate and a detailed activity mechanism of OGT needs to be further explored as it has role in increasing the efficacy of drug delivery.