Elevated Ozone Levels Affect Metabolites and Related Biosynthetic Genes in Tartary Buckwheat.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY(2020)
摘要
Global climate change and the industrial revolution have increased the concentration of tropospheric ozone, a photochemical air pollutant that can negatively affect plant growth and crop production. In the present study, we investigated the effects of O-3 on the metabolites and transcripts of tartary buckwheat. A total of 36 metabolites were identified by gas chromatography coupled with time-of-flight mass spectrometry, and principal component analysis was performed to verify the metabolic differences between nontreated and O-3 -treated tartary buckwheat. The content of threonic acid increased after 2 days of the O-3 treatment, whereas it decreased after 4 days of exposure, after which it gradually increased until the eighth day of exposure. In addition, the levels of most metabolites decreased significantly after the O-3 treatment. On the contrary, the levels of two anthocyanins, cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside, increased more than 11.36- and 11.43-fold, respectively, after the O-3 treatment. To assess the effect of O-3 on the genomic level, we analyzed the expression of anthocyanin biosynthesis pathway genes in O-3-treated and nontreated buckwheat using quantitative real-time reverse transcription polymerase chain reaction (PCR). We found that the expression of all anthocyanin pathway genes increased significantly in the O-3-treated buckwheat compared to that in the nontreated buckwheat. Altogether, our results suggested that O-3 affected the transcripts and metabolites of tartary buckwheat, which would eventually cause phenotypic changes in plants.
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关键词
anthocyanin,metabolome,ozone,tartary buckwheat,transcriptome
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