Occurrence And Enterotoxin Gene Profiles Of Staphylococcus Aureus Isolated From Retail Chicken Meat

The present study was conducted to evaluate the prevalence and enterotoxin gene profiles of Staphylococcus aureus isolated from 120 chicken meat marketed in retail outlets of Chennai, India. It was observed that total of 120 meat samples collected from different retail outlets, 66.67% (80/120) of the samples were positive for the presence of S. aureus based on biochemical characterization and species specific PCR based on thermonuclease gene (nuc). Enterotoxin gene profiling of the isolates for 9 genes (sea- sej) revealed that 52.50% (42/80) of the isolates in the present study were enterotoxigenic harboring either one or more gene. It was evident that majority of the isolates harbored seb, followed by seg, sei, sec, sed and sej either alone or in combination. None of the isolates harbored sea, see and seh either alone or in combination. The results of the study clearly indicated higher prevalence of enterotoxigenic S. aureus in retail meat marketed in Chennai, India indicating the potential of retail chicken meat to act as vehicle for food borne intoxication and a major public health threat.