Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing

Karen L. Nielsen,Paul A. Godfrey,Marc Stegger,Paal S. Andersen,Michael Feldgarden,Niels Frimodt-Møller

Journal of Microbiological Methods（2014）

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摘要

Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.

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关键词

Molecular typing,Whole-genome sequencing,Fecal flora,Escherichia coli,Urinary tract infection

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