Isolation, Purification and Identification of 20 – Hydroxymaytenin As a Bioactive Metabolite from Maytenus Heterophylla Liquid Cell Culture

20 – hydroxymaytenin belongs to the group of quinone - methide petacyclictriterpenes (QMTs), which showed a highly cytotoxicity in wide ranges of cancel cell lines. Therefore, they are pharmaceutical attractive metabolites[1]. However, not all of QMT derivatives are available commercially including 20 – hydroxymaytenin. So, the only way to obtain and used QMTs as a standard reference is to isolate from the plant material. Based on the previous studies, there are two main approaches are available. First method involves in the multiple steps of classical column chromatography[1] and second method is using the preparative HPLC with a suitable column[2]. These methods require either time to process or proper instruments. Here, we propose a short time procedure with regular and basic techniques to isolate and purify 20 – hydroxymaytenin.