Isolation, draft genome sequencing and identification of Enterobacter roggenkampii CCI9.

Strain CCI9, which was isolated from leaf soil collected in Japan, was capable of growth on poor-nutrient medium, at temperatures of 10°C to 45°C, at pHs of 4.5 to 10, and in the presence of 7.0% NaCl. We determined a draft genome sequence of strain CCI9, which consists of a total of 28 contigs containing 4,644,734 bp with a GC content of 56.1%. This assembly yielded 4,154 predicted coding sequences. Multilocus sequence analysis (MLSA) based on , , and gene sequences were performed to further identify strain CCI9. The MLSA revealed that strain CCI9 clustered tightly with EN-117. Moreover, the average nucleotide identity value (98.6%) between genome sequences of strain CCI9 and EN-117 exceeds the cutoff value for prokaryotic subspecies delineation. Therefore, strain CCI9 was identified as CCI9. To clarify differences between EN-117 and CCI9, the coding proteins were compared against the eggNOG database.