Study of Enterobacteria responsible to cause diarrhea in large animal and its drug resistance pattern

Nigella sativa L. is an annual herbaceous plant of the family Ranunculaceae. Seed being the economic part is the spice of commerce, composed of therapeutically potent metabolites. Owing to its wider application, the plants of Nigella sativa L. are widespread in Mediterranean, Middle European and South Asian countries. The present study is aimed at laying foundation for crop improvement and germplasm conservation programmes in Nigella sativa L. The results indicate that, plants upon germination takes 58 to 62 days for initiation of reproductive phase. Pollination activities starts 20 to 25 days later, upon anthesis an individual flower take one day for onset of male phase and on fifth day of anthesis the female part will be receptive. About seventy per cent of the pollens showed viability in terms of invitro germination in media composition of 45 % sucrose + 0.04 % boric acid. The phenotypic indices strongly reflecting the chronology of flowering events have been identified and illustrated, enabling the breeders and curators to assess and carryout the key activities of pollination.