Purification And Identification Of Surface Active Amphiphilic Candidates Produced Bygeotrichum Candidummk880487 Possessing Antifungal Property

The present study was held to optimize production, purify and identify biosurfactant from yeast strainGeotrichum candidumMK880487. Biosurfactant production was done on modified Hua medium and optimized in shake-flask method. Soybean oil (8%, v/v), KNO3(0.75 g L-1) and yeast extract (0.3 g L-1) were the best carbon and nitrogen sources, respectively, with C:N ratio of 200:1, pH 8 at 30 degrees C for 168 h. Production scale-up was achieved on 2.5 L bioreactor as batch method. The resulting crude biosurfactant extract with yield of 1.75 g L(-1)was purified by reversed phase column preparative HPLC. The resulting most active fraction F47 significantly reduced water surface tension by 51.92%. Chemical characterization using TLC, GC-MS, FTIR and LC-MS/MS methods revealed the biosurfactant to contain mainly glycolipid structure existing as mixture of Icariside F2, Cardenolide Di-Hexopyranoside and Di Galactosyl Di Acyl Glycerol glycolipid that collectively showed potential antifungal activity towardMacrophomina phaseolina.