The C-terminal Domain of piggyBac Transposase Is Not Required for DNA Transposition

•The C-terminal CRD in pble transposases is not essential for transposition.•Two CRD-deficient pble transposases trigger transposition of Ifp2.•Proper and improper insertions occur when CRD-deficient transposases mediate mobility.•CRD-deficient and full-length pble transposases do not insert transposons at random.•Features of the domesticated transposase PGBD5 originate from wild type transposase.