The effect of heat-treatment on SARS-CoV-2 viability and detection

•SARS-CoV-2 is not fully inactivated by heating to 56 °C or 60 °C for up to 60 min.•Complete inactivation occurred at 80 °C for 90 min or 95 °C for 1 or 5 min.•Heating SARS-CoV-2 to 80 °C for more than 30 min results in increased Ct values.•Significant variation in inactivation efficacy occurred between replicate samples.•Due to variability heat-inactivation protocols require local validation.