Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres

•A macroporous microspehres functionalized with peptide ligand and polymer brush.•Polymer brush helped to circumvent the steric effect of peptide ligand with antibody.•High dynamic binding capacity was maintained at high flow rate and short residence time.•Attractive candidate for rapid and low-cost purification of antibody.