Role of FoxO1 and insulin on cell proliferation mediated by Sirt1 in goose primary hepatocytes

Summary In order to explore the role of insulin and forkhead box protein O1 (FoxO1) on cell proliferation regulated by Sirt1 in goose primary hepatocyte, goose primary hepatocytes were isolated and incubated with Sirt1 inhibitor, nicotinamide, and then some hepatocytes were transfected with FoxO1 interference plasmid, or treated with insulin. The indexes related with cell proliferation were measured. The results firstly showed Sirt1 inhibition induced by nicotinamide increased cell proliferation in goose primary hepatocytes (P 0.05), protein content of p21 (P \u003e 0.05) and (PI) (67.40% vs 69.00%). Lastly, The PI of co-treatment with nicotinamide and insulin (56.65%) were lower than the single nicotinamide treatment (67.40%); the DNA synthesis rate in co-treatment of nicotinamide and insulin were lower than the single nicotinamide treatment (P