Human Blood Plasma Investigation Employing 2D UPLC-UDMS E Data-Independent Acquisition Proteomics.
Methods in molecular biology (Clifton, N.J.)(2021)
摘要
Proteomic tools are especially useful when it comes to investigating complex samples such as human blood plasma, in which protein quantities can span across up to ten orders of magnitude. Ultra definition mass spectrometry, in combination with two-dimensional liquid chromatography, provides better coverage of complex proteomes and allows for better control of collision energy, keeping the fragmentation benefits of high collision energy associated with drift time measurements from ion mobility separation. Here, we present a protocol to assist in the identification of proteins in human blood plasma and other similar samples with a large dynamic range.
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关键词
Depletion,Immunoaffinity depletion,Mass spectrometry,Multifactorial disorders,Serum,Tryptic digestion
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