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同型半胱氨酸对血管平滑肌细胞α1[Ⅰ]及α1[Ⅲ]型胶原mRNA表达的影响

JOURNAL OF JILIN UNIVERSITY(MEDICINE EDITION)(2004)

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Abstract
目的:观察同型半胱氨酸(Hcy)对血管平滑肌细胞(VSMCs)胶原合成的影响.方法:采用体外培养的大鼠VSMCs,加入不同浓度的Hcy(0、0.10、0.25、0.50和1.00mmol·L-1)作用后,通过测定培养基中羟脯氨酸含量和半定量RT-PCR法检测Hcy对VSMCs胶原合成及对α1[Ⅰ]、α1[Ⅲ]型胶原mRNA表达的影响.结果:Hcy促进VSMCs胶原合成及α1[Ⅰ]型、α1[Ⅲ]型胶原mRNA表达,并呈剂量依赖效应.结论:Hcy促进VSMCs胶原合成,可能是通过上调α1[Ⅰ]、α1[Ⅲ]型胶原mRNA表达实现的.
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