儿童急性淋巴细胞白血病患者HLA-A,B,DRB1等位基因多态性研究

目的研究北方汉族人群儿童急性淋巴细胞性白血病(ALL)患者与HLA-A,B,DRB1等位基因多态性的遗传关联.方法采用聚合酶链反应-序列特异性寡核苷酸探针(PCR-SSO)方法,对197例北方汉族儿童ALL患者和5841例健康脐带血样本HLA-A,B,DRB1等位基因多态性进行研究.结果在HLA-A,B,DRB1等位基因中,儿童ALL患者的A11(18 53%,x2=8.59,P＜0.01,RR=1.47,EF=0.0635)和A24(19 29%,x2=11.65,P＜0.01,RR=1.56,EF=0.0738);B40(18%,x2=1199.62,P＜0.001,RR=53.23,EF=0.18),B15(30%,x2=159.17,P＜0.001,RR=9.689,EF=0.014),B56(1.5%,x2=16.17,P＜0.001,RR=5.92,EF=0.0774),B67(2.5%,x2=5.93,P＜0.01,RR=2.74,EF=0.0774),和B27(3.75%,x2=4.96,P＜0.01,RR=1.81,EF=0.0774);DR9(18.21%,x2=16.07,P＜0.001,RR=1.74,EF=0.0774),DR12(14.13%,x2=7.12,P＜0.01,RR=1.523,EF=0.049),DR14(7.88%,x2=5.194,P＜0.05,RR=1.574,EF=0.0287),和DR16(4.89%,x2=8.527,P＜0.01,RR=2.065,EF=0.025)等基因的基因频率都显著高于正常人群.而HLA-B48(1.5%,x2=3.86,P＜0.05,RR=0.466,PF=0.0363),HLA-DR7(8.15%,x2=7.078,P＜0.01,RR=0.60,PF=0.086)和DR15(12 23%,x2=4.049,P＜0.05,RR=0.7109,PF=0.067)等基因的基因频率显著下降.结论 HLA-A11,A24,B15,B58,B67,B27,DR9,DR12,DR14,DR16等基因对儿童ALL患者有遗传易感作用,尤其是B40与ALL具有强相关性;而HLA-B48,DR7,DR15等基因对儿童ALL患者有遗传拮抗作用.