Cryogenic Ion Spectroscopy Of Adenine Complexes Containing Alkali Metal Cations

Cryogenic ion spectroscopy was used to characterize adenine complexes containing alkali metal cations (M(+)A, M = Cs, Rb, K, Na, and Li) produced by electrospray ionization. The ultraviolet (UV) photodissociation spectra of the complexes stored in a cryogenic ion trap exhibited well-resolved vibronic bands near their origin bands of the S-0-S-1 transition. The UV-UV hole-burning and infrared ion-dip spectra showed that all the M(+)A ions in the ion trap were single isomers of M(+)A7a, where the M+ ion was not bound to canonical 9H-adenine (A9) but bound to a rare tautomer, 7H-adenine (A7). Density functional theory calculations showed lower tautomerization barriers for M(+)A9 than for bare A9 in aqueous solution. We suggest that M+ ions not only play a catalytic role in the tautomerization of A9 to A7 but also increase the tautomerization yield by forming stable M(+)A7a isomers.