Multiplexing Viral Approaches to the Study of the Neuronal Circuits.
Journal of neuroscience methods(2021)
摘要
Neural circuits are composed of multitudes of elaborately interconnected cell types. Understanding neural circuit function requires not only cell-specific knowledge of connectivity, but the ability to record and manipulate distinct cell types independently. Recent advances in viral vectors promise the requisite specificity to perform true "circuit-breaking" experiments. However, such new avenues of multiplexed, cell-specific investigation raise new technical issues: one must ensure that both the viral vectors and their transgene payloads do not overlap with each other in both an anatomical and a functional sense. This review describes benefits and issues regarding the use of viral vectors to analyse the function of neural circuits and provides a resource for the design and implementation of such multiplexing experiments.
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关键词
Fluorophore,Genetically encoded calcium sensor,Neuronal tract tracing,Optogenetics,Opsin,Viral vector
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